With the continuous development of the biological industry, biological buffers have attracted more and more attention. As long as it is necessary to stabilize pH changes, they will be used. In other words, the role of the biological buffer is that when an appropriate amount of acid or alkali is added to other solutions, it can play a moderating role and ensure the stability of the pH value. Tris, as one of the more common buffers, has always appeared in major experiments, but some people still don't know it. Next, I will introduce it to you in detail.
The appearance of Tris is a white crystalline powder, which can be dissolved in ethanol, water and ethyl acetate, but when dissolved in ethyl acetate, it is relatively slow and has a corrosive effect on copper and aluminum.
Since Tris is weakly alkaline, when the ambient temperature is lower than 25 degrees, the Pka is 8.1. According to the buffer range, its effective buffer pH is between 7.0-9.2. However, the pH of the aqueous solution of Tris is around 10.5. At this time, it is necessary to fully adjust the pH value by adding hydrochloric acid to meet the requirements. During this period, you should also pay attention to the influence of the surrounding temperature, and you should not be in a temperature that is too high or too low.
Tris buffer is weak alkaline, deoxyribonucleic acid is deprotonated in the solution, so as to achieve the purpose of full dissolution. Because of the dissolution effect, Tris and EDTA can be used to configure TE buffer. If the pH-adjusted solution is replaced with acetic acid, TAE buffer can be obtained.
As an alkaline buffer, Tris can be widely used in acute respiratory acidosis and has a strong buffering effect on acidosis. However, at the same time, it should be noted that the use of professional guidance is required, not self-use. During use, it may cause hypoglycemia, hypotension, dizziness, nausea, and even shortness of breath, and be careful not to overdose or have impaired renal function. The able-bodied are not suitable for use.
Desheng Biochemical has been established for 10 years. It has its own unique insights and rich experience in the research and development of biological buffer series products. Tris, as the main product of Desheng biological buffer, has always been "paying close attention" to product quality. All products All inspections meet the qualified requirements before they can leave the factory. At present, it has been exported to more than 100 countries around the world, and has reached a cooperative relationship with it. The product quality is guaranteed, the price is negotiated according to the quantity, and the customer is the first. If you have this demand, please click on the official website or inquire!
